Validating a filter for microbiology

Several factors affect the measurement of a test solution's antimicrobial activity, and these must be considered in the validation design.They include the nature of the microorganisms used as challenge organisms, the preparation of the inoculum of challenge organisms, the specific conditions of the test, and the conditions of recovery.Chemical inhibition of bactericides is the preferred method for the antimicrobial efficacy test.

The growth and preparation of the challenge organism determines the physiological state of the cell.

shows known neutralizers for a variety of chemical antimicrobial agents and the reported toxicity of some chemical neutralizers to specific microorganisms.